ELAVL1 Polyclonal Antibody

Catalogue Numbers: BS60419-50, BS60419-100

Sizes: 50µl, 100µl

Alternative Name: ELAV-like protein 1; Hu-antigen R; HuR; ELAVL1; HUR

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15717

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The Elav-like genes encode for a family of RNA-binding proteins. Elav, a Drosophila protein and the first described member, is expressed immediately after neuroblastic differentiation into neurons and is necessary for neuronal differentiation and maintenance. Several mammalian Elav-like proteins, designated HuC, HuD and Hel-N1, are also expressed in postmitotic neurons. An additional mammalian homolog, HuR, which is also designated HuA, is ubiquitously expressed and is also overexpressed in a wide variety of tumors. Characteristically, these homologs all contain three RNA recognition motifs (RRM) and they specifically bind to AU-rich elements (ARE) in the 3'-untranslated region of mRNAs transcripts. ARE sites target mRNA for rapid degradation and thereby regulate the expression genes involved in cell growth and differentiation. When Elav-like proteins associate with these ARE sites this degradation is inhibited, leading to an increased stability of the corresponding transcript. Elav proteins function within the nucleus, and they are shuttled between the nucleus and cytoplasm by a nuclear export signal, which is a regulatory feature of the Elav-like proteins as it limits their accessibility to ARE sites.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ELAVL1 polyclonal antibody detects endogenous levels of ELAVL1 protein.

Bioworld Molecular Weight: ~ 36 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of Jurkat cell lines, using ELAVL1 antibody.

Immunogen: A synthetic peptide corresponding to residues in Human ELAVL1.

Conjugate: Unconjugated

Modification: Unmodified